Asbestos surface charge heterogeneity and biological effects.

نویسنده

  • W G Light
چکیده

T. Astrum, in Proteases and Biological Control, 77, 985 (1980). E. Reich, D. B. Rifkin, E. Shaw, Eds. (Cold 13. W. E. Laug, P. A. Jones, C. A. Nye, W. F. Spring Harbor Laboratory, Cold Spring Harbor, Benedict, Biochem. Biophys. Res. Commun. 68, N.Y., 1975), p. 343. 114 (1976). 5. N. W. Seeds, A. G. Gilman, T. Amano, M. W. 14. J. P. Quigley, J. Cell Biol. 71, 472 (1976). Nirenberg, Proc. Natl. Acad. Sci. U.S.A. 66, 15. P. H. Black, Adv. Cancer Res. 32, 76 (1980). 160 (1970). 16. K. M. Yamada, B. S. Spooner, N. K. Wessells, 6. B. Hamprecht, Int. Rev. Cytol. 49, 99 (1977). Proc. Natl. Acad. Sci. U.S.A. 66, 1206 (1970). 7. J. E. Bottenstein and G. H. Sato, Proc. Natl. 17. J.-D. Vassalli, J. Hamilton, E. Reich, Cell 8, 271 Acad. Sci. U.S.A. 76, 514 (1979). (1976). 8. P. R. Becherer and J. T. Wachsman J. Cell 18. J. C. Unkeless, A. Tobia, L. Ossowski, J. P. Physiol. 104, 47 (1980). Quigley, D. B. Rifkin, E. Reich, J. Exp. Med. 9. A. Krystosek, in preparation. 137, 85 (1973). 10. Anuran and chick sensory ganglia neurons ex19. A. G. Ware, M. M. Guest, W. H. Seegers, Arch. planted into culture dishes extend axons along Biochem. 13, 231 (1967). the substratum [R. G. Harrison, J. Exp. Zool. 9, 20. M. W. Mosesson, Biochim. Biophys. Acta 57, 787 (1910); D. Bray, Proc. Natl. Acad. Sci. 204 (1962). U.SA. 65, 905 (1970); M. A. Luduena, Dev. 21. Casein agarose and fibrin agarose overlay asBiol. 33, 268 (1973); P. C. Letourneau, ibid. 44, says were previously used to detect PA [A. R. 77 (1975)]. Elongation occurs at the growth Goldberg, Cell 2, 95 (1974); P. Jones, W. Benecone, which rapidly extends and retracts long dict, S. Strickland, E. Reich, ibid. 5, 323 (1975)]. microspikes. Axonal growth cones can also Agarose overlay thickness was approximately be seen in the developing nervous system by 400 iim in the center of the plate, increasing to using histological techniques [S. Ramon y Cajal, 2000 ,um at the edges (2.3 ml of substrate per 60Studies on Vertebrate Neurogenesis, L. Guth, mm dish). Typical development times for lytic Transl. (Thomas, Springfield, Ill., 1960)]. zones around transformed cell colonies were 12 Growth of neurites from neuroblastoma cells is to 16 hours at 37°C. More than 24 hours was qualitatively similar, in terms of the cellular needed to detect fibrinolysis by single NG108 processes involved, to that described for pricells. With the fibrin slide method clots had an mary neurons (5). average thickness of 15 ,um and could vary 11. G. A. Dunn, J. Comp. Neurol. 143, 491 (1971). above and below this value on the same cover 12. Fibroblasts of the European field vole (Microtus slip. Thickness of the overlay is decreased when agrestis) transformed by the Schmidt-Ruppin the clot is dehydrated for microscopic viewing. strain of avian sarcoma virus were the kind gift Partial fibrinolysis was detected after as little as of R. L. Erikson. Properties of the cells are 30 minutes, and incubations of 2'h to 3 hours described in Y. C. Chen, M. J. Hayman, P. K. were used for the examples shown in Fig. 1. Vogt, Cell 11, 513 (1977); M. S. Collett and R. L. Erikson, Proc. Natl. Acad. Sci. U.S.A. 75, 2021 5 May 1981

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عنوان ژورنال:
  • Science

دوره 213 4515  شماره 

صفحات  -

تاریخ انتشار 1981